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Teknova soytone agar plates
In vitro characterization of VNP20009-AF and its passages. a) viability: VNP20009 passages (P1–P5), VNP20009-AF, and post-freeze VNP20009-AF did not show a difference in bacterial survival when the frozen glycerol stocks were thawed out and serial dilutions were plated on <t>LB-Soytone</t> agar plates. Viability was measured as CFU/ml. b) API 20E identification test of wild type S . Typhimurium (14028), parental VNP20009, VNP20009 P1 and the animal-product-free VNP20009 (AF) showed only minor biochemical changes in the VNP20009-AF compared to parental VNP20009. c) motility assay on swimming agar plates showed that the wild type 14028, but not VNP20009 or VNP20009-AF, S . Typhimurium is motile. d) Gram stainings of VNP20009 and VNP20009-AF did not show morphological differences between the two strains.
Soytone Agar Plates, supplied by Teknova, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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In vitro characterization of VNP20009-AF and its passages. a) viability: VNP20009 passages (P1–P5), VNP20009-AF, and post-freeze VNP20009-AF did not show a difference in bacterial survival when the frozen glycerol stocks were thawed out and serial dilutions were plated on LB-Soytone agar plates. Viability was measured as CFU/ml. b) API 20E identification test of wild type S . Typhimurium (14028), parental VNP20009, VNP20009 P1 and the animal-product-free VNP20009 (AF) showed only minor biochemical changes in the VNP20009-AF compared to parental VNP20009. c) motility assay on swimming agar plates showed that the wild type 14028, but not VNP20009 or VNP20009-AF, S . Typhimurium is motile. d) Gram stainings of VNP20009 and VNP20009-AF did not show morphological differences between the two strains.

Journal: Human Vaccines & Immunotherapeutics

Article Title: Culture of attenuated Salmonella Typhimurium VNP20009 in animal-product-free media does not alter schwannoma growth control

doi: 10.1080/21645515.2023.2262639

Figure Lengend Snippet: In vitro characterization of VNP20009-AF and its passages. a) viability: VNP20009 passages (P1–P5), VNP20009-AF, and post-freeze VNP20009-AF did not show a difference in bacterial survival when the frozen glycerol stocks were thawed out and serial dilutions were plated on LB-Soytone agar plates. Viability was measured as CFU/ml. b) API 20E identification test of wild type S . Typhimurium (14028), parental VNP20009, VNP20009 P1 and the animal-product-free VNP20009 (AF) showed only minor biochemical changes in the VNP20009-AF compared to parental VNP20009. c) motility assay on swimming agar plates showed that the wild type 14028, but not VNP20009 or VNP20009-AF, S . Typhimurium is motile. d) Gram stainings of VNP20009 and VNP20009-AF did not show morphological differences between the two strains.

Article Snippet: Parental VNP20009 was cultured and serially passaged five times by growing in animal-product-free LB-containing Soytone medium (Becton Dickinson, Cat# 212488) and animal-product-free LB-containing Soytone agar plates (Teknova, Cat# L1000).

Techniques: In Vitro, Motility Assay